Next-generation sequencing using microfluidic PCR enrichment for molecular autopsy
نویسندگان
چکیده
منابع مشابه
A long PCR–based approach for DNA enrichment prior to next-generation sequencing for systematic studies1
UNLABELLED PREMISE OF THE STUDY We present an alternative approach for molecular systematic studies that combines long PCR and next-generation sequencing. Our approach can be used to generate templates from any DNA source for next-generation sequencing. Here we test our approach by amplifying complete chloroplast genomes, and we present a set of 58 potentially universal primers for angiosper...
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The enrichment of targeted regions within complex next generation sequencing libraries commonly uses biotinylated baits to capture the desired sequences. This method results in high read coverage over the targets and their flanking regions. Oxford Nanopore Technologies recently released an USB3.0-interfaced sequencer, the MinION. To date no particular method for enriching MinION libraries has b...
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Abstract DNA sequencing is one of the great valuable techniques in molecular biology, which can be used to detect the sequence of nucleotides in a DNA fragment. The high-throughput sequencing known as Next Generation Sequencing (NGS) revolutionized genomic research and molecular biology; therefore, the whole human genome can be sequenced with a low cost in several days. NGS technology is simi...
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ژورنال
عنوان ژورنال: BMC Cardiovascular Disorders
سال: 2019
ISSN: 1471-2261
DOI: 10.1186/s12872-019-1154-8